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At various intervals, they took MRI images of the mices discs and used a technique for imaging proteins called immunofluorescent staining to observe progress
For cell sorting, cell suspensions were stained with FITC-conjugated human Ep-CAM antibody and Alexa Fluor APC-conjugated human C-Kit for 30 minutes on ice
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Advantages: Combination with hyaluronic acid shows synergy
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