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Neelakantan and colleagues (Biochem Pharmacol, 2017) then took the pharmacological step, reporting that selective, membrane-permeable small-molecule NNMT inhibitors reversed high-fat-diet-induced obesity in mice this is the study most directly relevant to the 5-Amino-1MQ class, because it moves from the target matters to a drug against the target does something in a live animal. And again, it is a mouse study

doi: 10.1016/j.immuni.2023.02.015 164 LiYXuJChenWWangXZhaoZLiYet al

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However, in our experimental model with BRIN-BD11 cells, 18 h exposure to 50 nM Ex-4 did not induce significant changes in cell numbers (Fig