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For more intensive treatments like NAD+ therapy , sessions may last between 2 to 5 hours

Prior to transfection, 200 ng of siRNA and 3 l Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) were mixed and incubated at room temperature for 15 min in serum-free media, then added dropwise on top of the cells

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