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Ethanol metabolism, CYP2E1 induction, compromised antioxidant defenses, mitochondrial injury, activation of Kupffer and stellate cells, hypoxia, and iron overload can all contribute to the alcohol-induced oxidative environment

doi:10.1002/prot.24208

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PMID: 32958033

Negative controls were treated either with pre-immune serum instead of the primary antibody (Supplementary Figure 1A) or non-specific secondary antibody (goat anti-mouse IgG) (Supplementary Figure 1B)