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Furthermore, by targeting and eliminating senescent cells, which accumulate with age and contribute to inflammation and tissue dysfunction, FOXO4-DRI could help improve cardiac health for those suffering with heart disease
Nrf2 depletion in macrophages leads to increased foam cell formation, increases the M1 inflammatory phenotype with enhanced expression of pro-inflammatory monocyte chemoattractant protein-1 and interleukin-6, and aggravates atherosclerosis (244, 245)
Then, the sections were coated with an anti-CD31 antibody (Santa Cruz Biotechnology, Dallas, TX) at 4 C overnight, followed by incubation with an HRP-labeled secondary antibody (Gene Tech, Shanghai, China), and the sections were visualized with a DAB system (Gene Tech)
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