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Insecticide susceptibility assays Insecticide susceptibility assays were performed in rearing cages (30 30 60 cm) in a greenhouse in the Plant Growth Facility at Colorado State University under a photoperiod of 16L:8D and temperature of 2224 C

(C) Northern blot analysis of mRNA samples probed with radioactively labeled phzA2

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Then, we examined the off-target effects in four single-cell colonies of site 2, and no mutations were detected in the ten most likely off-target sites by Sanger sequencing (Supplementary Fig

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