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Samples were immersed in RNAlater stabilization solution (Thermo Fisher) and stored at 80 C until RNA extraction

EGCG remarkably attenuates neuronal apoptosis in the hippocampus induced by TBI by upregulating Bcl-2, downregulating Bax, and cleaved caspase-3 expression (Ye et al., 2023)

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10.1016/j.lfs.2021.119504 209 Ramos-VaraJ

For glutathione depletion in mice, BSO (Sigma) was dissolved in drinking water at a concentration of 20 mM

This pattern suggests DSIP's potential influence on the opioidergic system, possibly contributing to mechanisms of stress mitigation or adaptation