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The slight acidity of the solution (pH approximately 3.04.0 at 0.1% concentration) protonates basic amino acid residues on poorly water-soluble peptides, increasing their net positive charge and improving electrostatic interaction with the surrounding aqueous solvent a mechanism that dramatically increases the solubility of many lyophilised peptide preparations that resist dissolution in neutral or alkaline aqueous conditions

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Conversely, over drugged ADCs typically exhibit higher aggregation that can be associated with increased toxicity and side effects in patients

doi: 10.1080/09513590.2019.1689553 139 AbediniMGhaediEHadiAMohammadiHAmaniR