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The recommended approach is to aliquot the reconstituted solution into single-use portions before freezing, minimizing repeated thermal cycling of the main research stock

Jansen RS, Mahakena S, de Haas M, Borst P, van de Wetering K

Enteric pathogens often gain access to the body by altering the structure and function of tight junctions to increase permeability of the barrier via the secretion of proteases, which can cleave tight junction proteins or by altering the cytoskeleton [116]

Neuman, M

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