Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity by augmenting energy expenditure
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Subsequently, 250-m-thick slices of the hindbrain containing the AP were cut on a Vibratome (Leica) and immersed in a recovery solution (in mM: 3 KCl, 118 NaCl, 25 NaHCO 3 , 1.2 NaH 2 PO 4 , 2.5 Glucose, 7 MgCl 2 , 0.5 CaCl 2
Still tight but more workable
This experiment utilized fluorescent probes that targeted Gsto2 messenger ribonucleic acid (mRNA)
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