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llinger R, Wang H, Yamashita K, Wegiel B, Thomas M, Margreiter R, et al

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Briefly, 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (PC, Avanti Polar Lipids, 850457), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE, Avanti Polar Lipids, 850757), and liver L--phosphatidylinositol (Liver PI, Avanti Polar Lipids, 840042) in chloroform were mixed at a 5:2:1 (w/w) ratio for Figure 3d and Extended Data Figure 4l, or PC:PE were mixed at a 5:2 (w/w) ratio for Extended Data Figure 7f

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In addition, PFOS can induce autophagy activation with increased Beclin and LC3BII expression through reactive oxygen species (ROS)-mediated extracellular signalregulated kinase (ERK) activation, which is followed by impeded autophagic flux, increased lysosomal membrane permeability (LMP), and, consequently, RTC apoptosis

Injection site rotation matters too