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In rats exposed to 43 C, spermatogenic cell damage is mainly apoptotic after 3045 min, whereas longer exposure for 6090 min leads to more severe injury dominated by necrotic changes, suggesting a shift from apoptosis to necrosis under sustained heat stress (Kaushik et al., 2018)

Reported timelines vary

MTT Cell Proliferation and Cytotoxicity Assay Kit (batch number C0009S) was obtained from Beyotime

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