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coli ATCC25922 when supplementing the bacterial growth media (cation-adjusted Mueller-Hinton broth) with 10% ( v/v ) FBS

The LR3 modification (13-amino-acid N-terminal extension plus Glu3Arg substitution) reduces affinity for IGFBP-1 through IGFBP-6 by roughly 2 to 3 orders of magnitude compared to native IGF-1, according to biochemical binding studies

Vomund, S., Schfer, A., Parnham, M

Cryobiology 23 , 1427 (1986)

Unlike thalidomide, which showed submicromolar potency in doseresponse assays, we did not detect inhibition by rac -dHTC1 until it reached a concentration of 50 M (Fig