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Arginines role is more straightforward: it serves as a substrate for nitric oxide production via endothelial nitric oxide synthase, with human studies confirming acute increases in plasma arginine but variable downstream effects on performance

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Accordingly, we combined the most effective reversion positions with Glu72Arg and applied them to the X(GD_L)aX(GD_L)c background to generate X(GD_L)dX(GD_L)f (Fig