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Incubate 1 hour at 37C

Cartoon schematic of a GST-fusion protein

Since we found a down regulation of pro-angiogenic factors VEGF, CXCL8 in PCa cells and chemokine receptors (CXCR1/2, CXCR4, CCR2) on endothelial cells, we assessed whether ALCAR 1 or 10 mM, would affect PCa cellc pro-angiogenic products release and consequently interfere with the ability of endothelial cells to functionally induce cell morphogenesis

There is no human clinical data establishing a timeline for gut-specific effects

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6 This lactylated HMGB1 is released from macrophages via exosome secretion, which increases endothelium permeability, thereby exacerbating polymicrobial sepsis progression