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It remains uncertain whether distinct initiation mechanisms trigger the inflammatory response by acting on priming signaling pathways, or if a reduction in the negative regulation of NLRP3 activation amplifies the inflammatory cascade
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Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
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Gastrointestinal events were the most common adverse events in both groups and generally occurred during dose escalation
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