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Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol

Extracellular ATP detection B16 cells were inoculated in the density of 1 10 5 cells each well in 24-well plates overnight, and then treated with different formulations (10 M)

By targeting IL-1, interleukin-18 (IL-18), and type I interferon pathways, probiotics can fine-tune inflammasome activity, thereby suppressing aberrant inflammatory responses while simultaneously strengthening host antiviral defenses (236)

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