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J Micromech Microeng

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Calcium/phosphate/DNA precipitates were prepared by mixing 200 L of plasmid DNA (16 g, pGL4.12- Bdnf-pIV :phRL-TK(int) = 10:1) in 250 mM CaCl 2 solution with an equal volume of 2 HEPES buffered saline (42 mM HEPES [pH 7.03], 274 mM NaCl, 9.5 mM KCl, 2.67 mM Na 2 HPO 4 , and 15 mM glucose) and then incubating at room-temperature for 15 min