Total reduction in spot ultraviolet, spot polarization, and skin tone were measured and recorded using a Janus Facial Analysis System (PIE Co., Ltd, Suwon-si, Gyeonggi-do, Korea)
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Studies revealed that when parameters are well managed to extend the contact period of sludge and increase the intensity of mixing, it will be possible to ensure the removal efficiencies in the oxidative ditch that is at par with or even higher than that which is observed in conventional activated sludge systems [56]
After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications (61)
Moore TC, Newmister SA, Rayment I, Escalante-Semerena JC