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Next, the ELISA plate was washed with wash buffer and 90 L of Substrate Solution was added to each well, covered with plate sealer, and allowed to incubate for 20 min at 37 C while in the dark

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Melanotan 2 (MT-2) is a synthetic cyclic heptapeptide analogue of alpha-melanocyte-stimulating hormone (alpha-MSH)

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High-fiber foods also help stimulate the release of GLP-1, which is beneficial for regulating appetite