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Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

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Talanta 182:4954 Ayaslioglu E, Erkek E, Oba AA, Cebecioglu E (2005) Doxycycline-induced staining of permanent adult dentition