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Western blotting Cells were harvested and suspended in Total Lysis Buffer (0.125 M Tris-HCl pH 6.8, 4% SDS and 20% glycerol)
doi:10.1186/s12933-018-0740-x
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Every unit on that insulin syringe corresponds to a precise milligram quantity, and getting it wrong means either underdosing (wasting time) or overdosing (wasting comfort)
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