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This is helpful because it bypasses your digestive system, where B12 absorption often breaks down due to gut health issues or low stomach acid
For pilot studies, method development, or any protocol that will not consume 100mg inside a sensible in-use window, the smaller vial is the technically preferable choice rather than merely the cheaper one
Seelig MS
Best practices usually include: Start with the lowest dose in the dosage range
Chromatographic separation was conducted on a Brownlee SPP C18 column (2.1 mm 75 mm, 2.7 m particle size, Perkin Elmer, Waltham, MA) using mobile phase A (100% H2O containing 0.1% formic acid and 1% of 1 M NH 4 OAc) and B (1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH 4 OAc)
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