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mRNA expression analysis by real-time PCR Total RNA was isolated from the dissected tissues using the RNeasy Mini kit (Qiagen, CA, USA), followed by reverse transcription using the High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturers instructions

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BMC cancer 18, 12301239

Because some of the things you might notice, the reduced interest in meals you cook together, the inability to finish food at restaurants, the declining enthusiasm for eating occasions that used to be enjoyable, are not about you

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Although many protective strategies have been evaluated, AIC still remains a significant threat and limitation in patients undergoing anthracycline therapy 8,9,10