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Consequently, this causes ischemic hypoxia and exacerbates stroke-associated brain edema and neuronal injury ( in vitro BBB model studies have reported tobacco-induced alterations in TJ protein expression and re-distribution, which increases intracellular ROS/RNS and the secretory profile of various pro-inflammatory markers ( Direct nicotine binding to nicotinic acetylcholine receptors on BECs induces acetylcholine-dependent nitric oxide (NO) release via activation of neurovascular endothelial NO synthase ( 2 enhances vascular membrane permeability at the BBB

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