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Using the Broad Institute's Connectivity Map (CMap), which compares gene expression signatures across thousands of compounds, Pickart et al

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Total cellular extracts (200 g of protein) were incubated with an anti-IKK- antibody (1 g/mL) in IP buffer overnight at 4 C on a rocker

VLDL-C, Very-Low-Density Lipoprotein-C