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Ice-cold MeOH (600 L), CHCl 3 (200 L), and H 2 O (500 L) samples were vortexed and then centrifuged (10,000 g , 10 min, 4 C) to precipitate proteins
5 Materials Reduced glutathione (GSH), oxidized glutathione (GSSG), 1-chloro-2,4-dinitrobenzene (CDNB), dithiotreitol (DTT), chemicals for Lowry solutions, S -hexylglutathione sepharose 6B, tert -butyl hydroperoxide (t-BOOH), EDTA, N -ethylmaleimide, 4-chloro-7-nitrobenzofurazan, 6-mercapto-1-hexanol, 3-bromopyruvate, bovine serum albumin (BSA) and all other reagents were purchased from Sigma-Aldrich (St
Are there alternative ways to increase glutathione levels
Future studies should consider a double-blind, placebo-controlled design (no screening round) to definitively determine the effect of a single round of DMSA on behavior
No significant difference between cases and controls was reported for GPx-1 activity in platelets [63]
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