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analysis (calcd., found for C 34 H 58 N 14 O 8 ): C (51.63, 51.62), H (7.39, 7.40), O (16.18,16.19), N (24.79, 24.80)

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For peptide 20-bound GCGR, its ICL2 moved downward and made extensive polar and nonpolar contacts with the binding groove formed by the N helix, 1 strand and 5 helix of G s , resulting in an ICL2G s interface area of 799 2 , significantly larger than that of GLP-1R (396 2 ) or GIPR (416 2 ) (Fig