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A 20 l mix, containing 8 l of cDNA sample, 10 l of SYBR Green PCR Master Mix (Thermo Fisher Scientific), and 2 l of 10 mM forward primer and reverse primer mixture was used to performed Quantitative RT-PCR with a StepOne Plus Real-time PCR system (Applied Biosystems, Foster City, CA, United States)
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Authors contributions Herry Sonjaya, Erni Damayanti, and Hikmayani Iskandar designed the study and wrote the draft article
Toppo, S., Floh, L., Ursini, F., Vanin, S
Walline CC, Blum JS, Linton T, Mangiacarne D, Liangpunsakul S
Science 285 , 13931396 (1999)
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