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4 L cleaving agent was formulated in a volume ratio of TFA:thioanisole:anisole:EDT=90:5:3:2, and pre-frozen for 2 h, and then poured into the 5 L reaction kettle, allowing to react for 2 h at the room temperature
A80-229AP, Bethyl Laboratories, Inc.) in PBS/T/M was added for 1 h at room temperature, followed by wash and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)
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High-Dose Vitamin E High-dose vitamin E can increase bleeding risk and interact with glucose-affecting medications
