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Imaging was performed by using the Leica DMi8 TIRF Widefield Fluorescence of 10 objective lens (Leica, Germany) and Zeiss Spinning Disc Axio Observer Z1 of 63 objective lens (Carl Zeiss) microscopes

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Pickart estimated the toxic dose for humans at approximately 22,500 mg, which sits roughly 3,750 times higher than a typical weekly therapeutic dose