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The interaction between the RING domain of E3 ubiquitin ligase and Pelo on the sperms promotes E3-mediated ubiquitinated degradation of Pelo through the K48-linked polyubiquitination via the 26 S proteasome pathway, which also synergistically causes the degradation of its binding partner Hbs1 (Fig

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Finally, we wondered whether the pool of Go targeting the ONM would keep its functionality