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Third, major differences existed between the respective trial protocols

Water can be contaminated by leaking or failing septic systems, runoff and flooding that carry waste into a well, cracks in the well casing, a damaged or loose well cap, and surface water seeping in around a poorly sealed wellhead

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Histopathological examination using H&E and AB-PAS staining revealed severe pathological changes in the colonic epithelium of DSS-treated mice