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Cells were resuspended in FACS buffer and intracellular fluorescence intensity was analyzed using flow cytometry

Of the factors that participate in the TSS pathway, cystathionine beta-synthase (CBS) and cystathionine -lyase (CSE) mediate the first two rate-limiting steps of TSS by condensing serine with homocysteine to cystathionine and then cysteine

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Treatment with 5-Amino-1MQ combined with diet transition produced dramatic whole-body adiposity and weight loss, rapidly normalising these measures to levels comparable to age-matched lean control animals an outcome that diet switch alone was unable to achieve in the same time frame

Avoid excessive sun exposure and strenuous activity for optimal absorption