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In brief, 6,250 cells were seeded into 96-well white cell culture-treated plates (Falcon, 353296), treated for 24 h as indicated and labelled following the manufacturers instructions
Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks
However, its future trajectory in clinical medicine hinges on overcoming significant hurdles and navigating a complex scientific and regulatory landscape
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