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Bogdandi, V
After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample
General cell culture and imaging The HeLa cell line used in this study was purchased from American Type Culture Collection (ATCC) and grown in DMEM (Gibco, 11965) media supplemented with 10% FBS and 1% 1003 Pen Strep (Gibco)
Human acute myeloid leukemia cell line MOLM13 GFP Luc , 27 was cultured in Roswell Park Memorial Institute (RPMI) with 10% (v/v) FBS (Gemini Bio-Products), 1% penicillin/streptomycin and 2 mM glutamine
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