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By 1955, British chemist Dorothy Crowfoot Hodgkin (1910-1994) of Cambridge University determined the molecular structure of cyanocobalamin using computer-assisted x-ray crystallography, work for which she received the 1964 Nobel Prize in Chemistry (139)

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The cell pellets were sonicated (peak power: 75, duty factor: 20, cycle: 200) and then transferred to a new tube and centrifuged at full speed for 20 min at 4 C

2001;280:L54754