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So, it strictly adheres to protocol, aka, Title 17 regulations

BMC Gastroenterology, 17, 96

Heart rate over 120 beats per minute

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All cell lines were treated with a fixed dose of MTX (25 nM) for 24 hours, as this dose was representative of MTXs average IC50 at 96 hours across all cell lines (ie, 18.4 nM)