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Treatment of phLF and phBEC with TGF-1, TNF-, and menadione For treatment of phLF, cells were seeded at a density of approximately 20,000 cells/cm 2 , starved for 24 hours in DMEM/F12 with 0.5% FBS and 100 nM sodium selenite, followed by treatment with 2 ng/ml TGF-1 (R&D Systems, Minneapolis, MM, USA), 10 ng/ml TNF- (PeproTech, Rocky Hill, NJ, USA), and 10 M menadione (Sigma-Aldrich) in starvation medium for the indicated time points
In the bladder, the glutathione adduct can be cleaved to acrolein and glutathione in a well-known reversible Michael addition
Our findings also show that MTX upregulates JAK1/STAT3 protein levels, which subsequently enhances the release of inflammatory cytokines, including TNF-, IL-1, and IL-6
DeAngelis, R
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