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Depletion of GSH as a consequence of acetaminophen toxicity to the liver has attracted the most attention in the medical scientific community, as it can frequently be fatal or require a liver transplant or emergency treatment to prevent liver failure (the liver is the organ with the greatest concentration of GSH)

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Although metabolites can be measured directly via liquid chromatography-mass spectrometry, this technique often requires strict sample storage conditions and is vulnerable to target degradation

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