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The cell suspension was centrifuged at 14,000 g for 10 min, and 3 l of supernatant was applied to an Accucore 150 Amide HILIC HPLC column (100 2.1 mm, 2.6 m particle size) equipped with a guard cartridge (at 30 C)

doi: 10.1111/j.1751-1097.1970.tb06017.x 67 KomendaJ.SobotkaR

Subsequently, PCA dimensionality reduction was carried out using the RunPCA function, and clustering was completed using the FindNeighbors and FindClusters functions with the resolution parameter set to 0.1

Ruttkay-Nedecky, B

Better yet, track a four-week rolling average rather than individual data points