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RT-qPCR assay To investigate the gene expression levels of Pelo, Hbs1, E3, and Hsp70 in insect vectors, the total RNAs of 50 intact insects infected with RGDV or treated with dsRNAs were extracted using TRIzol reagent (Invitrogen) following the manufacturers instructions

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Juli 2013

The vegans averaged 0.76 grams of protein per kilogram of body weight per day (roughly 10% of daily calories from protein), while the Calorie Restriction members averaged 1.73 grams (roughly 24% of calories from protein)

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Approximately 34 g of each sample was immediately transferred into the devices stainless steel measurement chamber, and the water activity (aw) value was recorded by directly reading the digital display [37]