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The membranes were incubated overnight at 4C with 5% skim milk in SuperBlock blocking buffer-blotting in Tris-buffered saline

It was found that the proportion of CD4 + T cells in mouse tumor tissues increased from 42.1% to 55.5% after NP4 treatment, while the proportion of CD8 + T cells increased from 19.0% to 28.9% (Fig

These distinctions were central to its development as a targeted metabolic peptide and continue to inform its use as a pharmacological reference in fat-metabolism research today

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