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The assay for -glutamylcysteine synthetase activity is based on the formation of GSH from cysteine, glutamate and glycine in the presence of the excess of glutathione synthetase
Cani PD, Delzenne NM: Interplay between obesity and associated metabolic disorders: new insights into the gut microbiota
(June 2006)
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(2) depleted hepatic glutathione levels and its intermediates despite unchanged level of reactive oxygen species, suggesting glutathione consumption exceeds production due to systemic oxidative stress after AKI
The researchers didnt feed glutathione to the yeast, but rather used evolutionary engineering to produce a specific strain that tends to produce more glutathione, as well as a glutathione-derived metal chelating compound called PC2 (phytochelatin 2)
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