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Gs recruitment assay G protein recruitment to CTR or individual AMYR subtypes was measured in COS-7 cells (ATCC, CRL-1651) transfected with c-myc-hCTRaleu-Rluc8:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP1 Nluc:Gs-Venus:G1:G2, c-myc-hCTRaleu:RAMP2-Nluc:Gs-Venus:G1:G2 or c-myc-hCTRaleu:RAMP3-Nluc:Gs-Venus:G1:G2 at a ratio of 2:0.1:1:1:1, in a total of 51 ng DNA per well using PEI at a ratio of 1:6 of DNA to PEI
Practice proper preparation and sanitation techniques
Analyst 139, 54395450
Upregulation of TREM2 expression promotes the transition of microglia from M1 to M2 type, enhancing their phagocytic function, reducing the release of inflammatory mediators, and inhibiting the inflammatory cascade response
The NIH ImageJ software was used to assess cell area and fibrotic area
Liu Y, Beyer A, Aebersold R
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