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These were cultured with Dulbeccos modified Eagles medium (D5796, Sigma-Aldrich, Saint-Louis, MO) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, and 100 g/mL streptomycin (Nacalai Tesque)

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It was also noted that the half lives for the liposome entrapped antioxidants were also markedly prolonged in comparison to the injection of free enzymes

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