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In general, it is estimated that a minimum dose of 300 mg per day is required, and that the maximum dose may reach 600 mg for the most serious conditions
The nuclei were counterstained using DAPI (Thermo Fisher Scientific), after which the stained sections were imaged with a confocal microscope (LSM880, Zeiss)
Int Heart J
Funding This study was supported by the National Natural Science Foundation of China to QZ (No.32270407) and the open fund of State Key Laboratory of Southwestern Chinese Medicine Resources (No.SCMR202103) and Natural Science Foundation of Sichuan (No.2022NSFSC0583) to JL
However, for most people with normal B12 levels, supplementation or injections do not significantly affect metabolism or fat loss
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