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Eluted peptides were desalted using trap column (1 mm 15 mm, Acclaim PepMap300 C18, 5 m, Thermo Fisher Scientific) for 5 min at a flowrate of 200 L/min and 0.1% formic acid as mobile phase
Myo-inositol has fewer side effects (especially gastrointestinal), but metformin has a stronger evidence base and is included in international guidelines
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The orange spectra are those acquired from the TDC group, while the green spectra are those acquired from the ASD group
Use sterile, single-use aliquots
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