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MS1 and MS2 accuracy, and retention time window scans, were set to 0 allowing DIA-NN to perform their automatic inference using first run in the experiment (DIA-NN recommended MS1 mass accuracy setting: 12.7639 ppm)

For IL-4, we found no effects of treatment, we did however find that the levels of this cytokine were higher in females than males (main effect of sex: F (1,48) = 6.954, p 2 = 0.127, p = 0.011

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Transferrin receptor expression in normal, iron-deficient and iron-overloaded rats