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the isoelectric point (pI) is the pH at which net charge is zero, calculated by averaging the pKa values of the two ionizable groups flanking it

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Statistical significance was assessed using two-tailed paired t-test

In laboratory and pre-clinical research settings, peptides of this classification are studied for their involvement in: Peptide-mediated metabolic signalling pathways Experimental energy-regulation mechanisms Hormone-responsive receptor interactions in research systems Cellular signalling cascades relevant to metabolic regulation studies All references to biological activity are provided solely for academic and scientific context and do not imply safety, efficacy, or suitability for any non-research application